An IL-15 Superagonist, ALT-803, Enhances Antibody-Dependent Cell-Mediated Cytotoxicity Elicited by the Monoclonal Antibody NEO-201 Against Human Carcinoma Cells.

Structural basis of a potent human monoclonal antibody against Zika virus targeting a quaternary epitope.

A significant mechanism of motion for therapeutic antibodies is antibody-dependent cell-mediated cytotoxicity (ADCC). ALT-803 is an interleukin-15 superagonist complicated that enhances ADCC towards human carcinoma cells in vitro and exerts an antitumor exercise in murine, rat, and human carcinomas in vivo.

The authors investigated the capacity of ALT-803 to modulate ADCC mediated by the humanized IgG1 monoclonal antibody (mAb) NEO-201 towards human carcinoma cells.ALT-803 modulating exercise on ADCC mediated by NEO-201 was evaluated on a number of NEO-201 ligand-expressing human carcinoma cells.

Purified human pure killer (NK) cells from a number of wholesome donors had been handled with ALT-803 earlier than their use as effectors in ADCC assay. Modulation of NK cell phenotype and cytotoxic operate by publicity to ALT-803 was evaluated by circulation cytometry and gene expression evaluation.

ALT-803 considerably enhanced ADCC mediated by NEO-201. ALT-803 additionally upregulated NK activating receptors, antiapoptotic components, and components concerned in the NK cytotoxicity, in addition to downregulated gene expression of NK inhibiting receptors.

These findings point out that ALT-803 can improve ADCC exercise mediated by NEO-201, by modulating NK activation and cytotoxicity, suggesting a potential scientific use of ALT-803 together with NEO-201 for the remedy of human carcinomas.

An IL-15 Superagonist, ALT-803, Enhances Antibody-Dependent Cell-Mediated Cytotoxicity Elicited by the Monoclonal Antibody NEO-201 Against Human Carcinoma Cells.
An IL-15 Superagonist, ALT-803, Enhances Antibody-Dependent Cell-Mediated Cytotoxicity Elicited by the Monoclonal Antibody NEO-201 Against Human Carcinoma Cells.

In vivo mixture of human anti-envelope glycoprotein E2 and -Claudin-1 monoclonal antibodies for prevention of hepatitis C virus an infection.

Despite the improvement of direct-acting antivirals (DAAs), hepatitis C virus (HCV) an infection stays a serious trigger for liver illness and most cancers worldwide.

Entry inhibitors block virus host cell entry and, due to this fact, forestall institution of persistent an infection and liver illness. Due to their distinctive mechanism of motion, entry inhibitors present a sexy antiviral technique in organ transplantation.

In this examine, we developed an progressive strategy in stopping HCV an infection utilizing a synergistic mixture of a broadly neutralizing human monoclonal antibody (HMAb) focusing on the HCV E2 protein and a host-targeting anti-claudin 1 (CLDN1) humanized monoclonal antibody. An in vivo proof-of-concept examine in human liver-chimeric FRG-NOD mice proved the efficacy of the mixture remedy at stopping an infection by an HCV genotype 1b infectious serum.

While administration of particular person antibodies at decrease doses solely confirmed a delay in HCV an infection, the mixture remedy was extremely protecting. Furthermore, the mixture proved to be efficient in stopping an infection of major human hepatocytes by neutralization-resistant HCV escape variants chosen throughout liver transplantation, suggesting {that a} mixture remedy is suited to the neutralization of difficult-to-treat variants.

In conclusion, our findings counsel that the mixture of two HMAbs focusing on completely different steps of virus entry improves remedy efficacy whereas concurrently lowering remedy period and prices.

Our strategy not solely supplies a scientific perspective to make use of HMAb mixture therapies to forestall graft re-infection and its related liver illness however may additionally assist to alleviate the pressing demand for organ transplants by permitting the transplantation of organs from HCV-positive donors.

Structural basis of a potent human monoclonal antibody against Zika virus targeting a quaternary epitope.

Structural basis of a potent human monoclonal antibody against Zika virus targeting a quaternary epitope.

Zika virus (ZIKV) is a main human pathogen and member of the Flavivirus genus within the Flaviviridae household. In distinction to most different insect-transmitted flaviviruses, ZIKV additionally may be transmitted sexually and from mom to fetus in people.

During latest outbreaks, ZIKV infections have been linked to microcephaly, congenital illness, and Guillain-Barré syndrome. Neutralizing antibodies have potential as therapeutic brokers. We report right here a 4-Å-resolution cryo-electron microscopy construction of the ZIKV virion in advanced with Fab fragments of the potently neutralizing human monoclonal antibody ZIKV-195.

The footprint of the ZIKV-195 Fab fragment expands throughout two adjoining envelope (E) protein protomers. ZIKV neutralization by this antibody is presumably achieved by cross-linking the E proteins, which probably prevents formation of E protein trimers required for fusion of the viral and mobile membranes.

A single dose of ZIKV-195 administered 5 days after virus inoculation confirmed marked safety against lethality in a stringent mouse mannequin of an infection.

 

Characterization of a extremely particular monoclonal antibody against human aldo-keto reductase AKR1C3.

Human aldo-keto reductase AKR1C3 (kind 2 3α-hydroxysteroid dehydrogenase/kind 5 17β-hydroxysteroid dehydrogenase) is concerned in testosterone and estrogen metabolism.

AKR1C3 expression is comparatively low in most tissues and excessive in prostate and mammary glands in regulating androgen and estrogen ranges. However, in lots of cancers, overexpression of AKR1C3 was noticed, thus prompting the event of therapeutics targeting AKR1C3. To facilitate the event of AKR1C3 targeting therapeutics, evaluating the expression of AKR1C3 is important.

As AKR1C3 is very homologous with its household proteins, AKR1C1, AKR1C2, AKR1C4 and different AKR1 proteins, reagents that may unambiguously discriminate these enzymes are wanted. In this report, a extremely particular monoclonal antibody for AKR1C3, 10B10, was developed and characterised.

Compared to different AKR1C3 antibodies, 10B10 is very particular and delicate to AKR1C3 in a number of assay codecs. Thus, 10B10 can be a useful software for the medical improvement of AKR1C3 targeting therapeutics and the research of AKR1C3 biology.

Double Lock of a Human Neutralizing and Protective Monoclonal Antibody Targeting the Yellow Fever Virus Envelope.

Yellow fever virus (YFV), a lethal human pathogen, is the prototype of the genus Flavivirus. Recently, YFV re-emerged in Africa and Brazil, resulting in tons of of deaths, with some instances imported to China. Prophylactic or therapeutic countermeasures are urgently wanted. Previously, a number of human monoclonal antibodies against YFV have been screened out by phage show.

Here, we discover that one of them, 5A, reveals excessive neutralizing efficiency and good safety. Crystallographic evaluation of the YFV envelope (E) protein in its pre- and post-fusion states reveals conformations much like these noticed in different E proteins of flaviviruses.

Furthermore, the buildings of 5A in advanced with the E protein in each states are resolved, revealing an invariant recognition web site. Structural evaluation and useful knowledge counsel that 5A has excessive neutralization efficiency as a result of it interferes with virus entry by stopping each virus attachment and fusion. These findings can be instrumental for immunogen or inhibitor design.

The monoclonal antibody GNbAC1: targeting human endogenous retroviruses in multiple sclerosis.

The monoclonal antibody GNbAC1: targeting human endogenous retroviruses in multiple sclerosis.

Multiple sclerosis (MS) is an autoimmune demyelinating dysfunction of the central nervous system (CNS). Despite enhancements of immunomodulatory therapies in relapsing-remitting MS, the pathomechanisms of progressive illness are poorly understood and therapeutically addressed to this point.

A pathophysiological function for proteins encoded by human endogenous retroviruses (HERVs) has been proposed. GNbAC1 is a monoclonal antibody directed in opposition to the envelope protein of a HERV with postulated involvement in MS.

This assessment addresses the therapy idea of GNbAC1, the design, preclinical and scientific growth of the antibody, as printed by November 2018. All 4 in-human trials (of which two addressed MS) are mentioned.The therapy idea of GNbAC1 is interesting however stays controversial as a result of conflicting outcomes relating to the hypothesized underlying pathomechanism.

Anticipated immunomodulatory results weren’t noticed in scientific or pharmacodynamic analyses of the at present obtainable information. However, a magnetic resonance imaging signal suitable with the remyelinating potential of GNbAC1 inspired additional growth of this antibody in progressive MS. No related points with tolerability or security have been described to this point.

The monoclonal antibody GNbAC1: targeting human endogenous retroviruses in multiple sclerosis.
The monoclonal antibody GNbAC1: targeting human endogenous retroviruses in multiple sclerosis.

Potent anti-influenza H7 human monoclonal antibody induces separation of hemagglutinin receptor-binding head domains.

Seasonal influenza virus infections could cause vital morbidity and mortality, however the risk from the emergence of a brand new pandemic influenza pressure might need doubtlessly much more devastating penalties. As such, there may be intense curiosity in isolating and characterizing potent neutralizing antibodies that concentrate on the hemagglutinin (HA) viral floor glycoprotein.

Here, we use cryo-electron microscopy (cryoEM) to decipher the mechanism of motion of a potent HA head-directed monoclonal antibody (mAb) sure to an influenza H7 HA. The epitope of the antibody shouldn’t be solvent accessible in the compact, prefusion conformation that typifies all HA buildings to this point.

Instead, the antibody binds between HA head protomers to an epitope that should be partly or transiently uncovered in the prefusion conformation. The “respiration” of the HA protomers is implied by the publicity of this epitope, which is in line with metastability of sophistication I fusion proteins.

This construction probably subsequently represents an early structural intermediate in the viral fusion course of. Understanding the extent of transient publicity of conserved neutralizing epitopes additionally could result in new alternatives to fight influenza that haven’t been appreciated beforehand.

Ferritin and Charcoal stripped Sera for cell culture

Cell culture Flasks

Cell culture grade serum with ferritin Stripped witch charcoal.

These sera are for normal human cell culture or for the culture of cell lines. We guarantee sterility of the serum through our extensive filtering system with 0.05 mesh filters and sterile clean room iso13485, iso9001 and Fda packaging norms.

Human Ferritin depleted/stripped serum (Ferritin
Alpha Diagnostics 1000 ml
Normal Human Plasma, charcoal stripped (steroid free)
Alpha Diagnostics 10 ml

Charcoal will remove interference due to the carbon binding to fatty molecules like hormones, cytokines, androgen’s, estradiols, progesterone, cortisol, testosterone, triiodothyronine (T3), thyroxine (T4). from FBS.

Activated Charcoal Decontamination Bag
Biotium 25ST < span>153.00 EUR
AMIES BROTH WITH CHARCOAL
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Fetal Bovine Serum   Fetal bovine serum (FBS) is the liquid fraction of clotted blood from fetal calves, depleted of cells, fibrin and clotting factors, but containing a large number of nutritional and macromolecular factors essential for cell growth. Bovine serum albumin is the major component of FBS. Growth factors in FBS are essential for the maintenance and growth of cultured cells. FBS also contains a variety of small molecules like amino acids, sugars, lipids, and hormones. FBS is used in a wide range of applications. One of the primary uses of FBS is in eukaryotic cell culture, where it provides many essential nutrients and growth factors that facilitate cell survival and proliferation. However, it is important to note that FBS in human cell cultures may introduce research artifacts; human cells cultured with human sera behave differently from those cultured with FBS [3]. FBS is also used in the research, manufacture, and control of human and veterinary vaccines and of biotech drugs, and used to stop trypsin digest or to serve as a protectant in cryopreservation [5]. Cell culture media without any serum have been in use for many years. Fetal bovine serum might not be the best supplement for cell culture.

Exosome-depleted FBS Media Supplement Heat Inactivated - USA Certified      
SBI 250 ml < span>893.00 EUR
Exosome-depleted FBS Media Supplement Heat Inactivated - USA Certified      
SBI 50 ml < span>255.00 EUR
Fetal Bovine Serum (FBS), Premium, 100% US Origin, ESC Tested
101Bio NULL < span>NULL EUR
Fetal Bovine Serum (FBS), Premium, 100% US Origin, ESC Tested
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Fetal Bovine Serum (FBS), Premium, 100% US Origin, ESC Tested
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Human Anti-Fetal Bovine/Calf Serum (FBS /FCS) IgG ELISA Kit, 96 tests, Quantitative
Alpha Diagnostics 1 Kit < span>773.00 EUR
Rabbit Anti-Fetal Bovine Serum (FBS) protein IgG-Agarose affinity matrix to remove fetal bovine serum
Alpha Diagnostics 2 ml < span>469.00 EUR

For example, bovine serum albumin with insulin-transferrin-sodium selenite and/or epidermal growth factor in the culture medium improves bovine embryo quality and trophoblast invasion as compared to fetal bovine serum. Why is fetal serum used vs. for example, newborn serum or adult serum?  

Fetal serum contains more growth factors:

Transferrin human
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Transferrin antibody
Fitzgerald 100 ug < span>349.00 EUR
Transferrin antibody
Fitzgerald 100 ul < span>393.00 EUR
and has lower gamma globulin (i.e., antibodies) content and non-fetal serum. These are important because the growth factors facilitate cell survival and proliferation while antibodies could bind to the cells in culture. In addition, fetal serum contains lower levels of complement proteins (complements) than those from adults or newborns. These complements have the undesirable effects of living cells in culture and interfering with immunoassays. What is the difference between serum and plasma?   Serum and plasma are both derived from whole blood by using centrifugation to remove components including red blood cells. The difference between plasma and serum is that coagulation proteins are present in plasma but have been removed from serum. Plasma is generally prepared by adding anti-coagulants to the blood before centrifugation, but the clotting proteins are not removed. Serum is prepared by allowing the blood to coagulate before centrifugation or by extensive/progressive centrifugation. Thus fibrinogen and proteins associated with clotting are not present in serum.

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Fibrinogen antibody
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Fibrinogen antibody
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Fibrinogen antibody
Fitzgerald 1 mg < span>514.00 EUR
Fibrinogen antibody
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Fibrinogen antibody
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In humans, the following 22 proteins comprise 99% of the total protein content of serum and plasma: albumin, total IgG, transferrin, fibrinogen, total IgA, alpha-2-macroglobulin, total IgM, alpha-1-antitrypsin, C3 complement, haptoglobin, alpha-1-acid glycoprotein, apolipoprotein-B, apolipoprotein-A1, lipoprotein (a), factor H, ceruloplasmin, C4 complement, complement factor B, pre-albumin, C9 complement, C1q complement, and C8 complement [7, 8]. The remaining 1% includes hundreds of proteins. The most abundant serum protein, albumin, at 50 mg/ml, comprises about half of the total protein mass. How is fetal bovine serum tested before being packaged and sold?   The International Serum Industry Association (ISIA; www.serumindustry.org), a trade organization of serum providers, established guidelines for quality control standardization. These guidelines require the following tests be performed using specific methodologies, and the test results must be available as the Certificate of Analysis Test bacteria and fungi – sterility testing mycoplasma cytopathic agents – viral testing hem adsorbing agents – viral testing bovine virus diarrhea – viral testing pH measurement Osmolality total protein, determined by the Biuret method Endotoxins hemoglobin electrophoretic patte performance testing, such as stem cell culture radial immunodiffusion immunoglobulin gamma-glutamyl transferase (GGT) Table 1. Minimum serum test requirements. Endotoxin testing Endotoxins, also called lipopolysaccharide (LPS) or lipooligosaccharide (LOS), comes from the outer membranes of Gram-negative bacteria and contributes to the clinical manifestations of a variety of pathogenic Gram-negative bacteria. In vivo, endotoxins induce fever and the inflammatory response while in cell culture they introduce variation in cellular responses. Endotoxin levels are measured by the limulus amebocyte lysate (LAL) test. Amebocytes are analogous to white blood cells in vertebrates and LAL is prepared from the blood of Limulus Polyphemus (American horseshoe crab;  1). LAL is very sensitive to endotoxins and coagulates in the presence of even a minute amount of endotoxin. The conservation status of the American horseshoe crab has been assessed [10] and discussed in popular culture [11]. Many of the horseshoe crab’s populations are at risk and thus care should be taken to protect them from loss. Fetal Bovine Serum

  1. Horseshoe crabs.

Specialty sera and other treatment We discuss some of commonly used specialty area below. Serum preparations dedicated to specific research topics, such as lipoprotein-deficient serum from Kalen Biomedical for cholesterol research [12], or for specialized gene expression systems, such as tetracycline-screened fetal bovine serum [13], are not discussed. FBS depleted of exosomes or other components Regular FBS contains a large number of extracellular vesicles, some of which are exosomes [14, 15]. When performing exosome research with cultured cells, it is critical to use FBS without exosomes. However, it is important to be aware that exosome-depleted FBS may affect and support cell growth differently than regular FBS [16, 17]. Exosome-depleted FBS can be either purchased directly from several commercial suppliers or prepared through either ultracentrifugation or ultrafiltration, though the effects of the depletion processes and/or the commercial sources may not be consistent in terms of cell growth and physiology Other components of FBS can be depleted, depending on the experimental requirements. For example, Galmozzi A et al depleted heme from FBS with ascorbic acid Heat inactivation A common treatment of FBS is heat-inactivation, where FBS is heated at 56°C for 30 minutes in a water bath with occasional shaking. The purpose is to inactivate whatever components of the complement system are present in the FBS [20], and other potential unknown inhibitors of cell growth. Previously heat-inactivation was also intended to remove mycoplasma contamination, which is no longer an issue since all serum products are now filtered through pore sizes small enough to remove mycoplasma. It must be noted that heat-inactivation may have undesired effects as well, such as reducing the ability of cultured cells to attach to surfaces Several suppliers have urged users not to heat-inactivate FBS for most cell culture needs. In each case it is advisable to evaluate the need for heat inactivation in a particular application, since different cells have different responses towards heat-inactivation of FBS Furthermore, like all other protocols with regard to FBS, the heat inactivation process must be done with care, since a temperature too high or prolonged heating will inactivate growth factors and generate precipitates. Aikawa T et al cultured mouse primary glial cells on poly-D-lysine-coated flasks in DMEM containing 10% heat-inactivated FBS from MilliporeSigma (F2442) [26]. Feldman D et al cultured HEK293FT cells in DMEM supplemented with heat-inactivated fetal bovine serum from Seradigm (97068-085), and A549, HCT116, HT1080, A375 cells with heat-inactivated fetal bovine serum from Sigma (F4135) to evaluate optical pooled screens [13]. Kulkai S et al maintained Hut-78 cells with heat-inactivated fetal bovine serum from Atlanta Biologicals Charcoal treatment Activated carbon can bind to lipophilic molecules and thus has been used to remove hormones such as androgen, estradiol, progesterone, cortisol, testosterone, triiodothyronine (T3), and thyroxine (T4) from FBS. These serum lipid hormones tend to interfere with immunoassay systems and insulin assay methods. Thermo Scientific HyClone (now GE Healthcare) charcoal stripped fetal calf serum (csFCS) was used to culture cells in order to identify TGF beta family members Dialysis Dialysis can remove all molecules with molecular weight less than 10,000 MW from FBS. This includes both polar and non-polar molecules. Hormones, cytokines, glucose, amino acids, and many others are removed. Dialysis also removes antibiotics and other exogenous molecules in FBS. Dialyzed FBS from Thermo Fisher Scientific was used in the culture of pancreatic ductal adenocarcinoma for SILAC mass spec study [29] and other SILAC studies [30] and in the culture of HeLa cells with Met analogue l-AHA pulse-chase for the click-chemistry detection of long-lived proteins Gamma-Irradiation Gamma irradiation can be part of the process to sterilize FBS. FBS is usually filtered through 0.1 um filters multiple times to eliminate microorganisms. Gamma irradiation can inactivate the viruses commonly present in bovine species. However, some viral species, such as parvovirus, are resistant to gamma-irradiation. Low IgG Though FBS has a quite low IgG content, even this level may be too high for some certain applications. The IgG level can be substantially reduced further by capture chromatography. FBS with low IgG is used for antibody production, recombinant protein synthesis and other applications. Stem cell culture Stem cell culture has stringent requirements in terms of growth factors. Some of the growth factors present in FBS promote stem cell differentiation. Several companies offer embryonic stem cell qualified FBS intended to maintain the undifferentiated stem cell. However, it is important to test specific lots of for their applicability in maintaining the pluripotency/totipotency of different types of stem cells. Country of origin Major cattle countries are the main providers of FBS. The countries include the US, Australia, New Zealand, Canada, several in South and Central Americas The production and collection of serum products are regulated by govement agencies. FBS is labeled as USDA-Grade or European-Grade. USDA-Grade FBS can be imported into any country free of bovine spongiform encephalopathy and foot and mouth diseases, while European-Grade FBS can be sold in most European and Asian countries How to take care of/store fetal bovine serum? FBS is best stored frozen, between -5 to -20°C, and can be thawed at a temperature between 2 to 8°C. It is often useful to aliquot and freeze the serum in smaller portions, often 50 ml tubes, to avoid many freeze and thaw cycles. Occasionally, some aliquots of FBS remain in a liquid at freezing temperatures. This is due to the lack of a nucleation center (particulate matter) for the crystallization (freezing) to start. If you simply flick the tubes with your finger, they usually solidify almost instantly. It is common to have some precipitate after thawing. The precipitates, likely due to denaturation of some of the serum proteins, can be cleared by brief centrifugation, and this generally does not affect the quality of the serum. Selection of fetal bovine serum Many suppliers provide FBS with different grades, country of origin, and treatment. FBS, itself, is a complex mixture, and variability between different grades, suppliers, and lots is to be expected. Thus, it is essential to establish a process for selecting and evaluating FBS. It is a good idea to examine the literature to determine how a particular selection of FBS was previously used in similar research or for the same cell culture and to pay close attention for potential lot-to-lot variation in the FBS. To assist with this, Labome has conducted a survey of FBS usage from scientific publications, discussed below, to help our visitors to select the most suitable FBS. Fetal Bovine Serum in the Literature Labome surveys literature for the materials used. Here the publications that cited fetal bovine serum are summarized. The publications are a random subset of over 10,000 publications. The major suppliers of FBS are Life Technologies (now part of Thermo Fisher Scientific), Thermo Fisher Scientific Hyclone (now part of GE LifeScience), and MilliporeSigma (Table 2). supplier brand new reference Thermo Fisher Gibco 94 100Thermo Fisher Gibco 94 10082-147 [31]; 16000-044 [32]; 10270-106 [33], [34]; GE LifeScience (was Thermo Fisher) Hyclone 47 [13, 35], SH30071 MilliporeSigma 31 F4135 [13], F2442 [37], F7524 Gemini Bio-Products 10 Atlanta Biologicals 8 [27], S11550 PAA Laboratories 7 [40] Omega Scientific 3 PAN Biotech 3 Paradigm Biological Industries 2 Wisent 1 081150 Coing 1 35-010- Altas Biologicals 1 VWR 1 89510-186 Biochrom 1 Suppliers and the numbers of publications citing their FBS (with catalog numbers, if available) in the surve Thermo Fisher Scientific / Life Technologies Life Technologies is a global biotechnology company headquartered in Carlsbad, Califoia. It’s formed in 2008 with the merger of Invitrogen Corporation and Applied Biosystems Inc. Many of their products are under the brand name Gibco. They provide cell culture products, such as cell culture medium, FBS, and other relevant reagents. The company merged with Thermo Fisher Scientific Fetal Bovine Serum Life Technologies Gibco

FBS from Life Technologies has been used to study the nucleolus as a phase-separated protein quality control entity in HEK293T and HeLa cells 

Pandolfini L et al cultured

HeLa/GFP Cell Line
Cell Biolabs 1 vial < span>572.00 EUR
HeLa/Cas9 Cell Line
Cell Biolabs 1 vial < span>572.00 EUR
Hela-RFP stable cells
GenTarget 2 x 106 cell/ml x 1ml < span>775.00 EUR
HeLa Nuclear Extract (DTT-free)
Biovision < span>185.00 EUR
HeLa Nuclear Extract (DTT-free)
Biovision < span>316.00 EUR
HeLa HCP Std. A-H
Cygnus Technologies 1 ml < span>488.00 EUR
Anti-HeLa HCP, Affinity Purified
Cygnus Technologies 1 mg < span>1913.00 EUR
HeLa Cell Lysate (Whole Cell)
Cusabio 200ug < span>150.00 EUR
Human Hela Whole Cell Lysate
BosterBio 200ug < span>150.00 EUR
Hela-GFP stable cells (Blasticidin)
GenTarget 2 x 106 cell/ml x 1ml < span>775.00 EUR
Hela-GFP stable cells (Puromycin)
GenTarget 2 x 106 cell/ml x 1ml < span>775.00 EUR
Hela-TetR (Puro) stable cells
GenTarget 2 x 106 cell/ml x 1ml < span>1210.00 EUR
HeLa Nuclear Extract (Epithelioid carcinoma cells)
Alpha Diagnostics 100 ug < span>164.00 EUR
HeLa Nuclear Extract (Epithelioid carcinoma cells)
Alpha Diagnostics 50 ug < span>128.00 EUR
Luciferase (firefly), Hela stable cells (Blasticidin)
GenTarget 2 x 106 cell/ml x 1ml < span>1210.00 EUR
and A549 cells in DMEM supplemented with 10% Gibco FBS (10270-106) and Caco-2 cells in Eagle’s Minimum Essential Medium supplemented with 20% Gibco FBS (10270-106) [33]. HEK293T cells are quite often maintained with 10% fetal bovine serum from Thermo Fisher GE Healthcare Hyclone (Hyclone was a brand from Thermo Fisher Scientific) GE Healthcare Hyclone FBS is widely used. De Cecco M et al used it at 15% to maintain the senescent fibroblasts LF1, IMR-90 and WI-38 in Ham’s F-10 media [35]. Nicetto D et al used Hyclone FBS (SH30071) to terminate trypsin digestion and maintain embryonic cells [4]. Laflamme C et al maintained HEK-293 cells in DMEM high-glucose from GE Healthcare (SH30081.01) containing 10% bovine calf serum from GE Healthcare (SH30072.03) \ Fetal Bovine Serum  \Permission from Thermo Fisher Scientific, the copyright owner. MilliporeSigma\ MilliporeSigma FBS products have been used to culture terminal Schwann cells [37], HEK293T cells (ATCC CRL-3216) and COS-7 cells (87021302) [38], the importance of the regulation of Matrimony protein levels to the oocyte-to-embryo transition in Drosophila

Ecdysoneless Homolog (Drosophila) (ECD) Antibody
Abbexa 100 ug < span>509.00 EUR
TH1-Like (Drosophila) (TH1L) Antibody
Abbexa 100 ug < span>509.00 EUR
TH1-Like (Drosophila) (TH1L) Antibody
Abbexa 400 ul < span>523.00 EUR
TH1-Like (Drosophila) (TH1L) Antibody
Abbexa 80 µl < span>286.00 EUR
ECSIT Homolog (Drosophila) (ECSIT) Antibody
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ECSIT Homolog (Drosophila) (ECSIT) Antibody
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Ecdysoneless Homolog (Drosophila) (ECD) Antibody
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  • < span>411.00 EUR
  • < span>1845.00 EUR
  • < span>599.00 EUR
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Drosophila melanogaster GAGA-POZ Protein
Abbexa
  • < span>230.00 EUR
  • < span>2332.00 EUR
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  • 10 ug
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ECSIT Homolog (Drosophila) (ECSIT) Antibody
Abbexa
  • < span>300.00 EUR
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Fer3-Like (Drosophila) (FERD3L) Antibody
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Recombinant Drosophila melanogaster GAGA-POZ
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Recombinant Drosophila melanogaster GAGA-POZ
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Recombinant Drosophila melanogaster GAGA-POZ
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Fer3-Like (Drosophila) (Ferd3l) Antibody
Abbexa 200 ul < span>384.00 EUR

the regulatory mechanisms of the stem cell properties of mouse mammary epithelial cells during development [48], the functional properties of the CK2 kinase in Drosophila [49], and the mechanism of PI3K gamma activation\ FBS from other suppliers Laflamme C et al cultured U2OS cells in DMEM high-glucose containing 10% tetracycline-free fetal bovine serum from Wisent (081150) [36]. Dong JX et al maintained primary hippocampal cultures on Matrigel from Coing with 5% fetal bovine serum from Atlanta Biologicals (S11550) [39]. Lee A, et al cultured Murine C2C12 myoblasts with DMEM supplemented with 10% fetal bovine serum from VWR (89510-186) [45]. Zhao N et al cultured U2OS cells from ATCC (HTB-96) in DMEM medium with 10% (v/v) fetal bovine serum from Altas Biologicals\Liu X et al cultivated murine 4T1 cells in DMEM supplemented with 10% fetal bovine serum from Biochrom \PromoCell FBS and Stemcell Technologies fetal calf serum were used in the experiments to analyze the cellular origin of adult functional blood vessels\ References

What is a Humanized Monoclonal Antibody for Cancer Research?

This mab is a combining a human antibody with a small part of a mouse or rcamel binder monoclonal. The mouse or camel attaches to the epitope, and the human part makes more friendly to the human immune system.

Camel monoclonal antibodies are developed at the VUB, Brussels by phage display of Alpaca Lymphocytes in E. coli cells.

Camel antibodies can also be used for X-ray diffraction protein crystallographic structure characterization

Today 7th of November 2019 the crystal structure of Insulin is still unknown. Roche spent over 50 million USD to try to identify the Crystal structure of Insulin but their research still did not reveil the unstable proteins structure.

With the Use of camel monoclonal antibodies we hope to be able to stabilize the structure and crystallize it long enough to get the X-ray spectroscopy measurement.