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Ferritin and Charcoal stripped Sera for cell culture

Cell culture Flasks

Cell culture grade serum with ferritin Stripped witch charcoal.

These sera are for normal human cell culture or for the culture of cell lines. We guarantee sterility of the serum through our extensive filtering system with 0.05 mesh filters and sterile clean room iso13485, iso9001 and Fda packaging norms.

Human Ferritin depleted/stripped serum (Ferritin
Alpha Diagnostics 1000 ml
Normal Human Plasma, charcoal stripped (steroid free)
Alpha Diagnostics 10 ml

Charcoal will remove interference due to the carbon binding to fatty molecules like hormones, cytokines, androgen’s, estradiols, progesterone, cortisol, testosterone, triiodothyronine (T3), thyroxine (T4). from FBS.

Activated Charcoal Decontamination Bag
Biotium 25ST 153.00 EUR
Description: Minimum order quantity: 1 unit of 25ST
Normal Human Plasma, charcoal stripped (steroid free)
Alpha Diagnostics 10 ml 164.00 EUR
Normal Human Plasma, charcoal stripped (steroid free)
Alpha Diagnostics 100 ml 408.00 EUR
Mask, 4-Layers Active Charcoal, 50/Pk
BBI Biotech 50UNIT 64.73 EUR
Sterile filtered chicken serum, 0.2 micron, charcoal stripped
Equitech 50 ml 163.80 EUR

Fetal Bovine Serum   Fetal bovine serum (FBS) is the liquid fraction of clotted blood from fetal calves, depleted of cells, fibrin and clotting factors, but containing a large number of nutritional and macromolecular factors essential for cell growth. Bovine serum albumin is the major component of FBS. Growth factors in FBS are essential for the maintenance and growth of cultured cells. FBS also contains a variety of small molecules like amino acids, sugars, lipids, and hormones. FBS is used in a wide range of applications. One of the primary uses of FBS is in eukaryotic cell culture, where it provides many essential nutrients and growth factors that facilitate cell survival and proliferation. However, it is important to note that FBS in human cell cultures may introduce research artifacts; human cells cultured with human sera behave differently from those cultured with FBS [3]. FBS is also used in the research, manufacture, and control of human and veterinary vaccines and of biotech drugs, and used to stop trypsin digest or to serve as a protectant in cryopreservation [5]. Cell culture media without any serum have been in use for many years. Fetal bovine serum might not be the best supplement for cell culture.

Products Not found.

For example, bovine serum albumin with insulin-transferrin-sodium selenite and/or epidermal growth factor in the culture medium improves bovine embryo quality and trophoblast invasion as compared to fetal bovine serum. Why is fetal serum used vs. for example, newborn serum or adult serum?  

Fetal serum contains more growth factors:

Transferrin Antibody
Abbexa 50 ml 648.00 EUR
transferrin fragment
ApexBio 1 mg 108.00 EUR
Description: Transferrin is the principal ironbinding protein in animal serum and is analogous in its iron-binding site and properties to lactoferrin1. Human transferrin is a single-chain glycoprotein of molecular weight near 80,000.
transferrin fragment
ApexBio 10 mg 340.00 EUR
Description: Transferrin is the principal ironbinding protein in animal serum and is analogous in its iron-binding site and properties to lactoferrin1. Human transferrin is a single-chain glycoprotein of molecular weight near 80,000.
and has lower gamma globulin (i.e., antibodies) content and non-fetal serum. These are important because the growth factors facilitate cell survival and proliferation while antibodies could bind to the cells in culture. In addition, fetal serum contains lower levels of complement proteins (complements) than those from adults or newborns. These complements have the undesirable effects of living cells in culture and interfering with immunoassays. What is the difference between serum and plasma?   Serum and plasma are both derived from whole blood by using centrifugation to remove components including red blood cells. The difference between plasma and serum is that coagulation proteins are present in plasma but have been removed from serum. Plasma is generally prepared by adding anti-coagulants to the blood before centrifugation, but the clotting proteins are not removed. Serum is prepared by allowing the blood to coagulate before centrifugation or by extensive/progressive centrifugation. Thus fibrinogen and proteins associated with clotting are not present in serum.

Fibrinogen Protein
Abbexa 2 mg 356.00 EUR
Fibrinogen Antibody
Abbexa
  • 398.00 EUR
  • 133.00 EUR
  • 1107.00 EUR
  • 537.00 EUR
  • 314.00 EUR
  • 100 μg
  • 10 μg
  • 1 mg
  • 200 μg
  • 50 μg
Fibrinogen Antibody
Abbexa
  • 453.00 EUR
  • 133.00 EUR
  • 1288.00 EUR
  • 620.00 EUR
  • 342.00 EUR
  • 100 μg
  • 10 μg
  • 1 mg
  • 200 μg
  • 50 μg
Fibrinogen Antibody
Abbexa 1 mg 815.00 EUR
Fibrinogen Antibody
Abbexa 0.5 mg 878.00 EUR
Fibrinogen Antibody
Abbexa 1 mg 843.00 EUR
Fibrinogen Antibody
Abbexa 1 mg 968.00 EUR
Fibrinogen Protein
Abbexa
  • 328.00 EUR
  • 6397.00 EUR
  • 230.00 EUR
  • 10 μg
  • 1 mg
  • 2 µg
Human Fibrinogen
Akron Biotech 350mg 0.00 EUR
anti-Fibrinogen
Abfrontier 50 ug 363.00 EUR
Description: Mouse polyclonal to Fibrinogen
anti-Fibrinogen
Abfrontier 50 ul 363.00 EUR
Description: Mouse polyclonal to Fibrinogen
anti-Fibrinogen
Abfrontier 50 ug 363.00 EUR
Description: Mouse polyclonal to Fibrinogen
anti-Fibrinogen
Abfrontier 100 ug 403.00 EUR
Description: Rabbit polyclonal to Fibrinogen
Fibrinogen (FG) Antibody
Abbexa
  • 314.00 EUR
  • 787.00 EUR
  • 411.00 EUR
  • 154.00 EUR
  • 258.00 EUR
  • 100 μg
  • 1 mg
  • 200 μg
  • 20 μg
  • 50 μg
Fibrinogen (FG) Antibody
Abbexa
  • 843.00 EUR
  • 439.00 EUR
  • 1 mg
  • 200 μg

In humans, the following 22 proteins comprise 99% of the total protein content of serum and plasma: albumin, total IgG, transferrin, fibrinogen, total IgA, alpha-2-macroglobulin, total IgM, alpha-1-antitrypsin, C3 complement, haptoglobin, alpha-1-acid glycoprotein, apolipoprotein-B, apolipoprotein-A1, lipoprotein (a), factor H, ceruloplasmin, C4 complement, complement factor B, pre-albumin, C9 complement, C1q complement, and C8 complement [7, 8]. The remaining 1% includes hundreds of proteins. The most abundant serum protein, albumin, at 50 mg/ml, comprises about half of the total protein mass. How is fetal bovine serum tested before being packaged and sold?   The International Serum Industry Association (ISIA; www.serumindustry.org), a trade organization of serum providers, established guidelines for quality control standardization. These guidelines require the following tests be performed using specific methodologies, and the test results must be available as the Certificate of Analysis Test bacteria and fungi – sterility testing mycoplasma cytopathic agents – viral testing hem adsorbing agents – viral testing bovine virus diarrhea – viral testing pH measurement Osmolality total protein, determined by the Biuret method Endotoxins hemoglobin electrophoretic patte performance testing, such as stem cell culture radial immunodiffusion immunoglobulin gamma-glutamyl transferase (GGT) Table 1. Minimum serum test requirements. Endotoxin testing Endotoxins, also called lipopolysaccharide (LPS) or lipooligosaccharide (LOS), comes from the outer membranes of Gram-negative bacteria and contributes to the clinical manifestations of a variety of pathogenic Gram-negative bacteria. In vivo, endotoxins induce fever and the inflammatory response while in cell culture they introduce variation in cellular responses. Endotoxin levels are measured by the limulus amebocyte lysate (LAL) test. Amebocytes are analogous to white blood cells in vertebrates and LAL is prepared from the blood of Limulus Polyphemus (American horseshoe crab;  1). LAL is very sensitive to endotoxins and coagulates in the presence of even a minute amount of endotoxin. The conservation status of the American horseshoe crab has been assessed [10] and discussed in popular culture [11]. Many of the horseshoe crab’s populations are at risk and thus care should be taken to protect them from loss. Fetal Bovine Serum

  1. Horseshoe crabs.

Specialty sera and other treatment We discuss some of commonly used specialty area below. Serum preparations dedicated to specific research topics, such as lipoprotein-deficient serum from Kalen Biomedical for cholesterol research [12], or for specialized gene expression systems, such as tetracycline-screened fetal bovine serum [13], are not discussed. FBS depleted of exosomes or other components Regular FBS contains a large number of extracellular vesicles, some of which are exosomes [14, 15]. When performing exosome research with cultured cells, it is critical to use FBS without exosomes. However, it is important to be aware that exosome-depleted FBS may affect and support cell growth differently than regular FBS [16, 17]. Exosome-depleted FBS can be either purchased directly from several commercial suppliers or prepared through either ultracentrifugation or ultrafiltration, though the effects of the depletion processes and/or the commercial sources may not be consistent in terms of cell growth and physiology Other components of FBS can be depleted, depending on the experimental requirements. For example, Galmozzi A et al depleted heme from FBS with ascorbic acid Heat inactivation A common treatment of FBS is heat-inactivation, where FBS is heated at 56°C for 30 minutes in a water bath with occasional shaking. The purpose is to inactivate whatever components of the complement system are present in the FBS [20], and other potential unknown inhibitors of cell growth. Previously heat-inactivation was also intended to remove mycoplasma contamination, which is no longer an issue since all serum products are now filtered through pore sizes small enough to remove mycoplasma. It must be noted that heat-inactivation may have undesired effects as well, such as reducing the ability of cultured cells to attach to surfaces Several suppliers have urged users not to heat-inactivate FBS for most cell culture needs. In each case it is advisable to evaluate the need for heat inactivation in a particular application, since different cells have different responses towards heat-inactivation of FBS Furthermore, like all other protocols with regard to FBS, the heat inactivation process must be done with care, since a temperature too high or prolonged heating will inactivate growth factors and generate precipitates. Aikawa T et al cultured mouse primary glial cells on poly-D-lysine-coated flasks in DMEM containing 10% heat-inactivated FBS from MilliporeSigma (F2442) [26]. Feldman D et al cultured HEK293FT cells in DMEM supplemented with heat-inactivated fetal bovine serum from Seradigm (97068-085), and A549, HCT116, HT1080, A375 cells with heat-inactivated fetal bovine serum from Sigma (F4135) to evaluate optical pooled screens [13]. Kulkai S et al maintained Hut-78 cells with heat-inactivated fetal bovine serum from Atlanta Biologicals Charcoal treatment Activated carbon can bind to lipophilic molecules and thus has been used to remove hormones such as androgen, estradiol, progesterone, cortisol, testosterone, triiodothyronine (T3), and thyroxine (T4) from FBS. These serum lipid hormones tend to interfere with immunoassay systems and insulin assay methods. Thermo Scientific HyClone (now GE Healthcare) charcoal stripped fetal calf serum (csFCS) was used to culture cells in order to identify TGF beta family members Dialysis Dialysis can remove all molecules with molecular weight less than 10,000 MW from FBS. This includes both polar and non-polar molecules. Hormones, cytokines, glucose, amino acids, and many others are removed. Dialysis also removes antibiotics and other exogenous molecules in FBS. Dialyzed FBS from Thermo Fisher Scientific was used in the culture of pancreatic ductal adenocarcinoma for SILAC mass spec study [29] and other SILAC studies [30] and in the culture of HeLa cells with Met analogue l-AHA pulse-chase for the click-chemistry detection of long-lived proteins Gamma-Irradiation Gamma irradiation can be part of the process to sterilize FBS. FBS is usually filtered through 0.1 um filters multiple times to eliminate microorganisms. Gamma irradiation can inactivate the viruses commonly present in bovine species. However, some viral species, such as parvovirus, are resistant to gamma-irradiation. Low IgG Though FBS has a quite low IgG content, even this level may be too high for some certain applications. The IgG level can be substantially reduced further by capture chromatography. FBS with low IgG is used for antibody production, recombinant protein synthesis and other applications. Stem cell culture Stem cell culture has stringent requirements in terms of growth factors. Some of the growth factors present in FBS promote stem cell differentiation. Several companies offer embryonic stem cell qualified FBS intended to maintain the undifferentiated stem cell. However, it is important to test specific lots of for their applicability in maintaining the pluripotency/totipotency of different types of stem cells. Country of origin Major cattle countries are the main providers of FBS. The countries include the US, Australia, New Zealand, Canada, several in South and Central Americas The production and collection of serum products are regulated by govement agencies. FBS is labeled as USDA-Grade or European-Grade. USDA-Grade FBS can be imported into any country free of bovine spongiform encephalopathy and foot and mouth diseases, while European-Grade FBS can be sold in most European and Asian countries How to take care of/store fetal bovine serum? FBS is best stored frozen, between -5 to -20°C, and can be thawed at a temperature between 2 to 8°C. It is often useful to aliquot and freeze the serum in smaller portions, often 50 ml tubes, to avoid many freeze and thaw cycles. Occasionally, some aliquots of FBS remain in a liquid at freezing temperatures. This is due to the lack of a nucleation center (particulate matter) for the crystallization (freezing) to start. If you simply flick the tubes with your finger, they usually solidify almost instantly. It is common to have some precipitate after thawing. The precipitates, likely due to denaturation of some of the serum proteins, can be cleared by brief centrifugation, and this generally does not affect the quality of the serum. Selection of fetal bovine serum Many suppliers provide FBS with different grades, country of origin, and treatment. FBS, itself, is a complex mixture, and variability between different grades, suppliers, and lots is to be expected. Thus, it is essential to establish a process for selecting and evaluating FBS. It is a good idea to examine the literature to determine how a particular selection of FBS was previously used in similar research or for the same cell culture and to pay close attention for potential lot-to-lot variation in the FBS. To assist with this, Labome has conducted a survey of FBS usage from scientific publications, discussed below, to help our visitors to select the most suitable FBS. Fetal Bovine Serum in the Literature Labome surveys literature for the materials used. Here the publications that cited fetal bovine serum are summarized. The publications are a random subset of over 10,000 publications. The major suppliers of FBS are Life Technologies (now part of Thermo Fisher Scientific), Thermo Fisher Scientific Hyclone (now part of GE LifeScience), and MilliporeSigma (Table 2). supplier brand new reference Thermo Fisher Gibco 94 100Thermo Fisher Gibco 94 10082-147 [31]; 16000-044 [32]; 10270-106 [33], [34]; GE LifeScience (was Thermo Fisher) Hyclone 47 [13, 35], SH30071 MilliporeSigma 31 F4135 [13], F2442 [37], F7524 Gemini Bio-Products 10 Atlanta Biologicals 8 [27], S11550 PAA Laboratories 7 [40] Omega Scientific 3 PAN Biotech 3 Paradigm Biological Industries 2 Wisent 1 081150 Coing 1 35-010- Altas Biologicals 1 VWR 1 89510-186 Biochrom 1 Suppliers and the numbers of publications citing their FBS (with catalog numbers, if available) in the surve Thermo Fisher Scientific / Life Technologies Life Technologies is a global biotechnology company headquartered in Carlsbad, Califoia. It’s formed in 2008 with the merger of Invitrogen Corporation and Applied Biosystems Inc. Many of their products are under the brand name Gibco. They provide cell culture products, such as cell culture medium, FBS, and other relevant reagents. The company merged with Thermo Fisher Scientific Fetal Bovine Serum Life Technologies Gibco

FBS from Life Technologies has been used to study the nucleolus as a phase-separated protein quality control entity in HEK293T and HeLa cells 

Pandolfini L et al cultured

HeLa Nuclear Extract (Epithelioid carcinoma cells)
Alpha Diagnostics 100 ug 164.00 EUR
HeLa Nuclear Extract (Epithelioid carcinoma cells)
Alpha Diagnostics 50 ug 128.00 EUR
HeLa Cell Lysate (Human epithelial Cell Line)
Abfrontier 200 ul 86.00 EUR
Description: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Human HeLa-S3 (Cervix Epithelioid Carcinoma) lysate
Alpha Diagnostics 100ug 238.00 EUR
Human HeLa Whole Cell Lysate, TNFa Stimulated
Alpha Diagnostics 100ug 213.00 EUR
Human HeLa (Cervix Adenocarcinoma) Cell Nuclear Extract
Alpha Diagnostics 100ug 225.00 EUR
Human HeLa (Cervix Adenocarcinoma) Whole Cell Lysate
Alpha Diagnostics 1 mg 524.00 EUR
cDNA from Human Tumor Cell Line: Hela
Biochain 40 reactions 376.00 EUR
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
p300 protein WB +ve control (HeLa nuclear extract)
Alpha Diagnostics 100 ul 286.00 EUR
Genomic DNA from Human Tumor Cell Line: Hela
Biochain 100 ug 243.00 EUR
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Total Protein from Human Tumor Cell Line: Hela
Biochain 1 mg 214.00 EUR
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Membrane Protein from Human Tumor Cell Line: Hela
Biochain 0.1 mg 285.00 EUR
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Total RNA from Human Tumor Cell Line: Hela
Biochain 50 ug 194.00 EUR
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
H2O2 stimulated HeLa Cell Lysate (Human epithelial cell line)
Abfrontier Each 100 ul 121.00 EUR
Description: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate - H2O2 stimulated
Human HeLa (Cervix Adenocarcinoma) Cell Nuclear Extract - Doxorubicin Stimulated
Alpha Diagnostics 100ug 225.00 EUR
and A549 cells in DMEM supplemented with 10% Gibco FBS (10270-106) and Caco-2 cells in Eagle’s Minimum Essential Medium supplemented with 20% Gibco FBS (10270-106) [33]. HEK293T cells are quite often maintained with 10% fetal bovine serum from Thermo Fisher GE Healthcare Hyclone (Hyclone was a brand from Thermo Fisher Scientific) GE Healthcare Hyclone FBS is widely used. De Cecco M et al used it at 15% to maintain the senescent fibroblasts LF1, IMR-90 and WI-38 in Ham’s F-10 media [35]. Nicetto D et al used Hyclone FBS (SH30071) to terminate trypsin digestion and maintain embryonic cells [4]. Laflamme C et al maintained HEK-293 cells in DMEM high-glucose from GE Healthcare (SH30081.01) containing 10% bovine calf serum from GE Healthcare (SH30072.03) \ Fetal Bovine Serum  \Permission from Thermo Fisher Scientific, the copyright owner. MilliporeSigma\ MilliporeSigma FBS products have been used to culture terminal Schwann cells [37], HEK293T cells (ATCC CRL-3216) and COS-7 cells (87021302) [38], the importance of the regulation of Matrimony protein levels to the oocyte-to-embryo transition in Drosophila

Notch Homolog 3 (Drosophila) Antibody
Abbexa
  • 732.00 EUR
  • 398.00 EUR
  • 150 μl
  • 50 μl
Patched Homolog 1 (Drosophila) Antibody
Abbexa
  • 732.00 EUR
  • 398.00 EUR
  • 150 μl
  • 50 μl
Pellino Homolog 1 (Drosophila) Antibody
Abbexa
  • 732.00 EUR
  • 398.00 EUR
  • 150 μl
  • 50 μl
Prickle Homolog 3 (Drosophila) Antibody
Abbexa
  • 732.00 EUR
  • 398.00 EUR
  • 150 μl
  • 50 μl
Prune Homolog 2 (Drosophila) Antibody
Abbexa
  • 732.00 EUR
  • 398.00 EUR
  • 150 μl
  • 50 μl
Pumilio Homolog 2 (Drosophila) Antibody
Abbexa
  • 732.00 EUR
  • 398.00 EUR
  • 150 μl
  • 50 μl
Salvador Homolog 1 (Drosophila) Antibody
Abbexa
  • 732.00 EUR
  • 398.00 EUR
  • 150 μl
  • 50 μl
Slingshot Homolog 3 (Drosophila) Antibody
Abbexa
  • 732.00 EUR
  • 398.00 EUR
  • 150 μl
  • 50 μl
Snail Homolog 2 (Drosophila) Antibody
Abbexa
  • 732.00 EUR
  • 398.00 EUR
  • 150 μl
  • 50 μl
Sonic Hedgehog Homolog (Drosophila) Antibody
Abbexa
  • 732.00 EUR
  • 398.00 EUR
  • 150 μl
  • 50 μl
Spire Homolog 1 (Drosophila) Antibody
Abbexa
  • 732.00 EUR
  • 398.00 EUR
  • 150 μl
  • 50 μl
Spire Homolog 2 (Drosophila) Antibody
Abbexa
  • 732.00 EUR
  • 398.00 EUR
  • 150 μl
  • 50 μl
Sprouty Homolog 2 (Drosophila) Antibody
Abbexa
  • 732.00 EUR
  • 398.00 EUR
  • 150 μl
  • 50 μl
Sprouty Homolog 3 (Drosophila) Antibody
Abbexa
  • 732.00 EUR
  • 398.00 EUR
  • 150 μl
  • 50 μl
Tribbles Homolog 2 (Drosophila) Antibody
Abbexa
  • 732.00 EUR
  • 398.00 EUR
  • 150 μl
  • 50 μl

the regulatory mechanisms of the stem cell properties of mouse mammary epithelial cells during development [48], the functional properties of the CK2 kinase in Drosophila [49], and the mechanism of PI3K gamma activation\ FBS from other suppliers Laflamme C et al cultured U2OS cells in DMEM high-glucose containing 10% tetracycline-free fetal bovine serum from Wisent (081150) [36]. Dong JX et al maintained primary hippocampal cultures on Matrigel from Coing with 5% fetal bovine serum from Atlanta Biologicals (S11550) [39]. Lee A, et al cultured Murine C2C12 myoblasts with DMEM supplemented with 10% fetal bovine serum from VWR (89510-186) [45]. Zhao N et al cultured U2OS cells from ATCC (HTB-96) in DMEM medium with 10% (v/v) fetal bovine serum from Altas Biologicals\Liu X et al cultivated murine 4T1 cells in DMEM supplemented with 10% fetal bovine serum from Biochrom \PromoCell FBS and Stemcell Technologies fetal calf serum were used in the experiments to analyze the cellular origin of adult functional blood vessels\ References

What is a Humanized Monoclonal Antibody for Cancer Research?

This mab is a combining a human antibody with a small part of a mouse or rcamel binder monoclonal. The mouse or camel attaches to the epitope, and the human part makes more friendly to the human immune system.

Camel monoclonal antibodies are developed at the VUB, Brussels by phage display of Alpaca Lymphocytes in E. coli cells.

Camel antibodies can also be used for X-ray diffraction protein crystallographic structure characterization

Today 7th of November 2019 the crystal structure of Insulin is still unknown. Roche spent over 50 million USD to try to identify the Crystal structure of Insulin but their research still did not reveil the unstable proteins structure.

With the Use of camel monoclonal antibodies we hope to be able to stabilize the structure and crystallize it long enough to get the X-ray spectroscopy measurement.